Difference between revisions of "Part:BBa K2596004"

Revision as of 04:38, 16 October 2018

PidiA from Synechococcus elongatus PCC 7942

This is a ferrous-ion repressible promoter for cyanobacteria Synechococcus elongatus PCC 7942.

Usage and Biology

PidiA is a ferrous-ion repressible promoter native to cyanobacteria. The source of the PidiA DNA is a close relative to our chassis (S. elongatus PCC 7942). The promoter features a palindromic repeat which is suggested to be activated by the binding of idiB, a gene immediately downstream of the idiA gene, in a ferrous deficient environment [3].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Our BioBrick has the 77bp full minimal idiA promoter sequence, plus an upstream AT-rich region [3]. We did not include downstream base pairs due to ambiguity in the true start codon [3].

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 <partinfo>BBa_K2596004 short</partinfo>
-ferric repressible
+This is a ferrous-ion repressible promoter for cyanobacteria Synechococcus elongatus PCC 7942.
-<!-- Add more about the biology of this part here
+<!-- Add more about the biology of this part here -->
 ===Usage and Biology===
+PidiA is a ferrous-ion repressible promoter native to cyanobacteria. The source of the PidiA DNA is a close relative to our chassis (S. elongatus PCC 7942). The promoter features a palindromic repeat which is suggested to be activated by the binding of idiB, a gene immediately downstream of the idiA gene, in a ferrous deficient environment [3].
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 <partinfo>BBa_K2596004 SequenceAndFeatures</partinfo>
+Our BioBrick has the 77bp full minimal idiA promoter sequence, plus an upstream AT-rich region [3]. We did not include downstream base pairs due to ambiguity in the true start codon [3].
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