Difference between revisions of "Part:BBa K2596003"
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<partinfo>BBa_K2596003 short</partinfo> | <partinfo>BBa_K2596003 short</partinfo> | ||
| − | This a | + | This a high-light inducible promoter from cyanobacteria Synechococcus elongatus PCC 7942 |
| − | <!-- Add more about the biology of this part here | + | <!-- Add more about the biology of this part here --> |
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | |||
| + | PpsbA2 is a light-inducible promoter native to cyanobacteria [6]. Specifically, it comes from a strain (Synechococcus elongatus PCC 7942) closely related to our chassis organism [7]. The promoter is activated by high light intensity or blue light, and deactivated by low light intensity or red light [8]. | ||
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<partinfo>BBa_K2596003 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2596003 SequenceAndFeatures</partinfo> | ||
| + | For the Biobrick, we kept most of the original promoter, including a crucial upstream element and the core promoter sequence. Our final Biobrick is 157 bp in length. The promoter contains a conserved AT-box motif just upstream of the ribosome binding site that serves as the binding site for light-activated proteins [9]. | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
Revision as of 04:14, 16 October 2018
PpsbA2 from Synechococcus elongatus PCC 7942
This a high-light inducible promoter from cyanobacteria Synechococcus elongatus PCC 7942
Usage and Biology
PpsbA2 is a light-inducible promoter native to cyanobacteria [6]. Specifically, it comes from a strain (Synechococcus elongatus PCC 7942) closely related to our chassis organism [7]. The promoter is activated by high light intensity or blue light, and deactivated by low light intensity or red light [8].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
For the Biobrick, we kept most of the original promoter, including a crucial upstream element and the core promoter sequence. Our final Biobrick is 157 bp in length. The promoter contains a conserved AT-box motif just upstream of the ribosome binding site that serves as the binding site for light-activated proteins [9].