Difference between revisions of "Part:BBa K2796028"
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<partinfo>BBa_K2796028 short</partinfo>
 
<partinfo>BBa_K2796028 short</partinfo>
 
<ul>   
 
<ul>   
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Considering that TIL cells don’t secret enough exosomes under normal condition, we fuse three genes to increase the number of exosomes (Alenquer, 2015), thus increasing the transfer efficiency of exosomes.<li><b>Exosome booster</b> refers to hSDC4-T2A-STEAP3-P2A-NadB.We identified <b>STEAP3</b> (involved in exosomebiogenesis), syndecan-4(<b>SDC4</b>; supports budding of endosomal membranes to form multivesicular bodies), and a fragment of L-aspartate oxidase  (<b>NadB</b>; possibly boosts cellular
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<li>Considering that <b>TIL</b> cells don’t secret enough exosomes under normal condition, we fuse three genes to increase the number of exosomes (Alenquer, 2015), thus increasing the transfer efficiency of exosomes.</li>
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metabolism by tuning up the citric acid cycle) as potential synthetic exosome production boosters. Combined expression of
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<li><b>Exosome booster</b> refers to hSDC4-T2A-STEAP3-P2A-NadB.We identified <b>STEAP3</b> (involved in exosomebiogenesis), syndecan-4(<b>SDC4</b>; supports budding of endosomal membranes to form multivesicular bodies), and a fragment of L-aspartate oxidase  (<b>NadB</b>; possibly boosts cellular metabolism by tuning up the citric acid cycle) as potential synthetic exosome production boosters. Combined expression of these genes significantly increased exosome production.(Ryosuke Kojima et al. 2008)</li>
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these genes significantly increased exosome production.(Ryosuke Kojima et al. 2008)</li>
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Here is the final device that we designed for this project.
 
Here is the final device that we designed for this project.
 
[[File:2018 LZU-CHINA vesicle attacker.png|600px|thumb|center|]]
 
[[File:2018 LZU-CHINA vesicle attacker.png|600px|thumb|center|]]

Revision as of 02:14, 16 October 2018


Exosome booster

  • Considering that TIL cells don’t secret enough exosomes under normal condition, we fuse three genes to increase the number of exosomes (Alenquer, 2015), thus increasing the transfer efficiency of exosomes.
  • Exosome booster refers to hSDC4-T2A-STEAP3-P2A-NadB.We identified STEAP3 (involved in exosomebiogenesis), syndecan-4(SDC4; supports budding of endosomal membranes to form multivesicular bodies), and a fragment of L-aspartate oxidase (NadB; possibly boosts cellular metabolism by tuning up the citric acid cycle) as potential synthetic exosome production boosters. Combined expression of these genes significantly increased exosome production.(Ryosuke Kojima et al. 2008)

    • Here is the final device that we designed for this project.

    2018 LZU-CHINA vesicle attacker.png

    It combines with exosome booster and miR attacker under control of induced promoter.More details and description you can obtain in our wiki!

    T--LZU-CHINA--design1.png

    Sequence and Features


    Assembly Compatibility:
    • 10
      COMPATIBLE WITH RFC[10]
    • 12
      COMPATIBLE WITH RFC[12]
    • 21
      INCOMPATIBLE WITH RFC[21]
      Illegal BglII site found at 192
      Illegal BglII site found at 1777
    • 23
      COMPATIBLE WITH RFC[23]
    • 25
      INCOMPATIBLE WITH RFC[25]
      Illegal AgeI site found at 2657
      Illegal AgeI site found at 3277
    • 1000
      INCOMPATIBLE WITH RFC[1000]
      Illegal SapI site found at 1367