Difference between revisions of "Part:BBa K2570008:Design"
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===Design Notes===
 
===Design Notes===
−
We ordered the part DNA from a synthesis company and had codon optimization of the sequence.
+
We have the part DNA synthesized from a gene synthesis company and had codon optimization of the sequence.
 +
 
 +
Get the target gene ''atoD'' by PCR.
 +
 
 +
Primers:
 +
 
 +
Fwd: 5’-aacggggtgatttatgaaaggagatataatgaaagttaccaatcagaaag-3’
 +
 
 +
Rev: 5’-cgaattcaccactagtaccagatctttaaaagcttttaatataaatatctttcag-3’
 +
 
  
  
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===References===
 
===References===
 +
[1] Anu Jose Mattam and Syed Shams Yazdan. Engineering E. coli strain for conversion of short chain fatty acids to bioalcohols. Biotechnol Biofuels. 2013; 6: 128.

Revision as of 01:58, 16 October 2018


adhE2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 916
    Illegal AgeI site found at 169
    Illegal AgeI site found at 571
    Illegal AgeI site found at 1008
    Illegal AgeI site found at 1942
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have the part DNA synthesized from a gene synthesis company and had codon optimization of the sequence.

Get the target gene atoD by PCR.

Primers:

Fwd: 5’-aacggggtgatttatgaaaggagatataatgaaagttaccaatcagaaag-3’

Rev: 5’-cgaattcaccactagtaccagatctttaaaagcttttaatataaatatctttcag-3’



Source

adhE2-aldehyde-alcohol dehydrogenase from Clostridium acetobutylicum ATCC 824

https://www.ncbi.nlm.nih.gov/gene/1116040

References

[1] Anu Jose Mattam and Syed Shams Yazdan. Engineering E. coli strain for conversion of short chain fatty acids to bioalcohols. Biotechnol Biofuels. 2013; 6: 128.