Difference between revisions of "Part:BBa K2570007:Design"
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===Design Notes===
 
===Design Notes===
 
The atoA sequence was obtained from ''E.coli Bw25113'' by PCR
 
The atoA sequence was obtained from ''E.coli Bw25113'' by PCR
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Primers:
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Fwd: 5’-gcaaataactcgagaaggagatataatggatgcgaaacaacgtattg-3’
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Rev: 5’-ttggtaactttcattatatctcctttcataaatcaccccgttgcg-3’
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[[File:T--FJNU-China--Basic_part-atoA_2.jpg|600px|thumb|center|Fig.1 PCR result of atoA.]]
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===Source===
 
===Source===

Revision as of 01:51, 16 October 2018


atoA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 180
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The atoA sequence was obtained from E.coli Bw25113 by PCR

Primers: Fwd: 5’-gcaaataactcgagaaggagatataatggatgcgaaacaacgtattg-3’

Rev: 5’-ttggtaactttcattatatctcctttcataaatcaccccgttgcg-3’

Fig.1 PCR result of atoA.


Source

Organism="Escherichia coli str. K-12 substr. MG1655". EC_number="2.8.3.9". Protein_id="AAC75281.1". https://www.ncbi.nlm.nih.gov/nuccore/U00096.3?from=2324109&to=2324759

References

Saini M, Chen M H, Chiang C J, et al. Potential production platform of n-butanol in Escherichia coli[J]. Metabolic Engineering, 2015, 27:76.