Difference between revisions of "Part:BBa K2614000"

Revision as of 22:59, 15 October 2018

template as positive control for a qPCR that detects Plasmodium

We developed a primer/probe set for a qPCR, which detects the human pathogens of the genus Plasmodium. This part can be used as positive control for our qPCR. If you like to run this qPCR you need one sense primer (CTGGCTAAACTTCCCAATGA), one antisense primer (GTCTATATTCATGTTTGATTGACCTT) and a dual labeled Probe ([6FAM]CTTcCAaATaGAtTTCGCAGAA[BHQ1] (lowercase letter = LNA)).

qPCR with primer/probe set for Plasmodium in general. BBa_K2614000 as positive control, cultured Plasmodium stems and negative control are shown.

Multiplex qPCR with primer/probe set for Plasmodium in general and Plasmodium falciparum. BBa_K2614000 as positive control, cultured Plasmodium stems and negative control are shown.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 We developed a primer/probe set for a qPCR, which detects the human pathogens of the genus Plasmodium. This part can be used as positive control for our qPCR. If you like to run this qPCR you need one sense primer (CTGGCTAAACTTCCCAATGA), one antisense primer (GTCTATATTCATGTTTGATTGACCTT) and a dual labeled Probe ([6FAM]CTTcCAaATaGAtTTCGCAGAA[BHQ1] (lowercase letter = LNA)).
 [[File:BBa_K2614000_qPCR_Plasmodium.png|200px|thumb|left|qPCR with primer/probe set for Plasmodium in general. BBa_K2614000 as positive control, cultured Plasmodium stems and negative control are shown. ]]
+[[File:BBa_K2614000_qPCR_multiplex.png|200px|thumb|left|Multiplex qPCR with primer/probe set for Plasmodium in general and Plasmodium falciparum. BBa_K2614000 as positive control, cultured Plasmodium stems and negative control are shown. ]]
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