Difference between revisions of "Part:BBa K2758000:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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In order to delay the expression of Killer Red protein relative to the accumulator (our other construct), we inserted an RBS with moderate binding capabilities into the plasmid of interest. Due to the fact that many of the parts used in this construct were too small to run through a gel after a double restriction and the lack of SpeI enzyme, our team ordered the sequence through IDT with the NotI restriction sites.
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This part is designed to work with another part that consists of a Hg accumulator. This part has been designed to express the Killer Red protein later than the accumulator is expressed from the other construct.  In order to delay the expression of Killer Red protein relative to the accumulator, we inserted an RBS with moderate binding capabilities into the K2758000 construct. We built this construct through IDT adding the NotI restriction sites.
  
 
===Source===
 
===Source===

Revision as of 22:12, 15 October 2018


Mercury activated Killer Red Protein suicide switch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 237
    Illegal BsaI.rc site found at 528


Design Notes

This part is designed to work with another part that consists of a Hg accumulator. This part has been designed to express the Killer Red protein later than the accumulator is expressed from the other construct. In order to delay the expression of Killer Red protein relative to the accumulator, we inserted an RBS with moderate binding capabilities into the K2758000 construct. We built this construct through IDT adding the NotI restriction sites.

Source

This sequence was constructed entirely with Biobricks from the iGEM registry. The sequences are: BBa_K346002 (mercury responsive promotor), BBa_B0030 (RBS), BBa_K1184000 (Killer Red), BBa_B0015 (double terminator).

References