Difference between revisions of "Part:BBa K2610013"
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iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry. | iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry. | ||
| − | We fused promoters to green fluorescent protein and stressed transformed | + | We fused promoters to green fluorescent protein and stressed transformed DH5α cells for several hours. |
[[File:T--Leiden--heatmap.png|750px]] | [[File:T--Leiden--heatmap.png|750px]] | ||
Latest revision as of 22:12, 15 October 2018
pUmuC
Mutagenesis proteins UmuC and UmuD, a part of the SOS system, are involved in the cellular response to DNA damage in E. coli (Reuven et al, 1999).
Usage and Biology
iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry. We fused promoters to green fluorescent protein and stressed transformed DH5α cells for several hours.
Figure 1. Heatmap with stress-activated promoters
As can be observed in Figure 1 the UmuC promoter is only moderately activated by tetracycline.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]