Difference between revisions of "Part:BBa K2758000:Design"

 
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===Design Notes===
 
===Design Notes===
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In order to delay the expression of Killer Red relative to the accumulator, we inserted an RBS with slow transcription. Due to the fact that many of the parts used in the construct were too small to run through a gel after a double restriction and the lack of SpeI enzyme, our team ordered the sequence through IDT with the NotI restriction sites.
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In order to delay the expression of Killer Red protein relative to the accumulator (our other construct), we inserted an RBS with moderate binding capabilities into the plasmid of interest. Due to the fact that many of the parts used in this construct were too small to run through a gel after a double restriction and the lack of SpeI enzyme, our team ordered the sequence through IDT with the NotI restriction sites.
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===Source===
 
===Source===

Revision as of 22:08, 15 October 2018


Mercury activated Killer Red Protein suicide switch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 237
    Illegal BsaI.rc site found at 528


Design Notes

In order to delay the expression of Killer Red protein relative to the accumulator (our other construct), we inserted an RBS with moderate binding capabilities into the plasmid of interest. Due to the fact that many of the parts used in this construct were too small to run through a gel after a double restriction and the lack of SpeI enzyme, our team ordered the sequence through IDT with the NotI restriction sites.

Source

This sequence was constructed entirely with Biobricks from the iGEM registry. The sequences are: BBa_K346002 (mercury responsive promotor), BBa_B0030 (RBS), BBa_K1184000 (Killer Red), BBa_B0015 (double terminator).

References