Difference between revisions of "Part:BBa K2610007"

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<partinfo>BBa_K2610007 short</partinfo>
 
<partinfo>BBa_K2610007 short</partinfo>
  
MicF small RNA, coded by the MicF gene, is produced as stress response in Escherichia coli. It negatively regulates translation of the OmpF porin. MicF binds to OmpF mRNA and represses production of OmpF in response to environmental and internal stresses. The MicF expression is stimulated by many factors such as oxidative stress, osmolarity increase and temperature increase.  
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MicF small RNA, coded by the MicF gene, is produced as stress response in Escherichia coli. It negatively regulates translation of the OmpF porin. MicF binds to OmpF mRNA and represses production of OmpF in response to environmental and internal stresses. The MicF expression is stimulated by many factors such as oxidative stress, osmolarity increase and temperature increase. <span class="plainlinks">[https://www.ncbi.nlm.nih.gov/pubmed/11601842 (Delihas et al, 2001]</span>
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 22:00, 15 October 2018


pMicF

MicF small RNA, coded by the MicF gene, is produced as stress response in Escherichia coli. It negatively regulates translation of the OmpF porin. MicF binds to OmpF mRNA and represses production of OmpF in response to environmental and internal stresses. The MicF expression is stimulated by many factors such as oxidative stress, osmolarity increase and temperature increase. (Delihas et al, 2001


Usage and Biology

iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry. We fused promoters to green fluorescent protein and stressed transformed DH5a cells for several hours.


T--Leiden--heatmap.png
Figure 1. Heatmap with stress-activated promoters

As can be observed in Figure 1 the MicFpromoter is only moderately activated by tetracycline

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]