Difference between revisions of "Part:BBa K2559001"

Revision as of 17:33, 15 October 2018

Bacterial cellulose synthase H (BcsH)

The BBa_K2559001 is coding a cellulose complement protein A (ccpA) .

Usage and Biology

It is encoded exclu-sively in the Komagataeibacter/Gluconacetobacter lineage and is required for the BCS activity in K. xylinus and Komagataeibacter hansenii It has been shown to interact with BcsD, and appears to assist the arrangement of glucan chains into crystalline ribbons. in the periplasm. This unique organization might account for the extremely high activity of the K. xylinus BCS.

The double digestion of biobrick with EcoRI and PstI

We introduced bcsZ, H, A, B, C, D, bglX from the Acetobacter xylinum which involve in the process of bacterial cellulose synthesis into the model organism cyanobacteria (Synechocystis sp.pcc6803),and we obtain the transgenic cyanobacteria.

Transgenic cyanobacteria with bcsZH-ABCD-bglX genes and the cellulose measurement

Cyanobacteria glucose content measurement (3 repeat). The same of quality of transferred cyanobacteria with bcsZH-ABCD-bglX gene and wildtype are treated with lysozyme to break out the cell. The glucose represents the content of cellulose. The addition of cellulase can digest the cellulose to glucose then the bacterial cellulose can measured. The distinct differences between the red column and blue column indicate the high expression intensity of bacteria cellulose. Wilcoxon test indicates our data is reliable.

The measurement of cellulose content in transgenic cyanobacteria which expressed bcs gene . The P-value verified that the distinction between treatment group and control group.

The part is facilitate the in-depth research for other teams!

Reference : 1.Romling U & Galperin MY (Bacterial cellulose biosynthesis: diversity of operons, subunits, products, and functions. (Translated from eng) Trends Microbiol 23(9):545-557 (in eng).

Sequence and Features