Difference between revisions of "Part:BBa K2680281:Design"
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===Design Notes=== | ===Design Notes=== | ||
To do | To do | ||
− | + | Sequence sent to William and Mary iGEM from Virginia iGEM. Visit their wiki [http://2018.igem.org/Team:Virginia/Parts here]. Used PCR to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary. | |
Revision as of 16:19, 15 October 2018
3G LsRK
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 109
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 41
Illegal BsaI.rc site found at 1647
Design Notes
To do Sequence sent to William and Mary iGEM from Virginia iGEM. Visit their wiki [http://2018.igem.org/Team:Virginia/Parts here]. Used PCR to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary.
Source
To do