Difference between revisions of "Part:BBa K2644002"
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<partinfo>BBa_K2644002 parameters</partinfo>
 
<partinfo>BBa_K2644002 parameters</partinfo>
 
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==Usage==
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Arsenic sensitive plasmid is a circuit which is sensitive to As3+ and express smURFP as a feedback so that we can indirectly educe As3+ concentration through detecting the fluorescent intensity of smURFP. It's composed of 4 small parts, including promoter J23104, RBS and ArsR gene(BBa_K2644010), promoter ArsR along with RBS(also named ArsR operator, BBa_K2644004), smURFP gene(BBa_K2644007) and HO-I gene(BBa_K2328062).

Revision as of 15:25, 15 October 2018


arsenic sensitive circuit

A plasmid which is sensitive to As3+ and can express fluorescent protein smURFP

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 198
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage

Arsenic sensitive plasmid is a circuit which is sensitive to As3+ and express smURFP as a feedback so that we can indirectly educe As3+ concentration through detecting the fluorescent intensity of smURFP. It's composed of 4 small parts, including promoter J23104, RBS and ArsR gene(BBa_K2644010), promoter ArsR along with RBS(also named ArsR operator, BBa_K2644004), smURFP gene(BBa_K2644007) and HO-I gene(BBa_K2328062).