Difference between revisions of "Part:BBa K2599016"

 
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<partinfo>BBa_K2599016 short</partinfo>
 
<partinfo>BBa_K2599016 short</partinfo>
  
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NCTU_Formosa 2018 designed this sequence to improve the part from NCTU_Formosa 2016.  
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We changed the general linker to a GS linker to optimize the function of Sf1a.
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Previous Part: BBa_K1974022
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The existing part from NCTU_Formosa 2016 contains the IPTG induced PT7 [https://parts.igem.org/Part:BBa_I712074 (BBa_I712074)], strong ribosome binding site [https://parts.igem.org/Part:BBa_B0034 (BBa_B0034)], Sf1a, linker, snowdrop lectin [https://parts.igem.org/Part:BBa_K1974020 (BBa_K1974020)] and the 6X His-Tag [https://parts.igem.org/Part:BBa_K1223006 (BBa_K1223006)].
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<p style="padding-top:20px;font-size:20px"><b>Introduction</b></p>
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Revision as of 15:05, 15 October 2018


T7 Promoter+RBS+Sf1a+GS linker+snowdrop-lectin+linker+6X His-Tag

NCTU_Formosa 2018 designed this sequence to improve the part from NCTU_Formosa 2016. We changed the general linker to a GS linker to optimize the function of Sf1a.


Previous Part: BBa_K1974022

The existing part from NCTU_Formosa 2016 contains the IPTG induced PT7 (BBa_I712074), strong ribosome binding site (BBa_B0034), Sf1a, linker, snowdrop lectin (BBa_K1974020) and the 6X His-Tag (BBa_K1223006).


Introduction


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]