Difference between revisions of "Part:BBa K2812004:Design"
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | DNA sequence codon optimized for E. coli. | + | Total DNA sequence has been codon optimized for E. coli. |
===Source=== | ===Source=== | ||
| − | + | The initial starting sequence for truncated lysostaphin was taken from <partinfo>BBa_K748002</partinfo>. The original sequence for the HlyA domain and thrombin linker were taken from uniprot. | |
| − | + | ||
===References=== | ===References=== | ||
Revision as of 13:12, 15 October 2018
Coding sequence for trunctated Lysostaphin fused to His-tagged HlyA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1351
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 126
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Total DNA sequence has been codon optimized for E. coli.
Source
The initial starting sequence for truncated lysostaphin was taken from BBa_K748002. The original sequence for the HlyA domain and thrombin linker were taken from uniprot.