Difference between revisions of "Part:BBa K2740015"
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<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
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<h2>Parameter of Protein </h2> | <h2>Parameter of Protein </h2> | ||
<p align="left">Number of amino acids: 509</p> | <p align="left">Number of amino acids: 509</p> | ||
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<p >[[File:T--Nanjing-China--nif K-structure.png|800px|thumb|center]]</p> | <p >[[File:T--Nanjing-China--nif K-structure.png|800px|thumb|center]]</p> | ||
<h2>Confirmation of Expression of nifK</h2> | <h2>Confirmation of Expression of nifK</h2> | ||
− | <p | + | <p>We test expression profiles of each structure gene in the nif cluster that overexpressed in EJNC by conducting Real-time Quantitative PCR(qPCR). Relative expression compared to the housekeeping gene 16S rRNA is shown.</p> |
− | [[File:T--Nanjing-China--nifK | + | [[File:T--Nanjing-China--nifK.jpg|800px|thumb|center]] |
− | + | <p>qRT-PCR analysis demonstrates that all the component genes of the nif cluster are significantly over expressed in EJNC whereas the transcription of these genes are no detected (N.D.) in nondiazotrophic E.coli JM109. Based on these analysis, we know nifK has a relatively low expression level.</p> | |
− | <p> | + | |
<div> | <div> | ||
<h2>Usage</h2> | <h2>Usage</h2> |
Revision as of 10:53, 15 October 2018
CR1 nifK
CR1 nifK encodes the subunit beta (NifK) of the molybdenum-iron protein. Together with NifD, they form a α2β2 heterotetramer.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 856
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1106
Illegal SapI.rc site found at 1489