Difference between revisions of "Part:BBa K2818000"

Revision as of 10:01, 15 October 2018

∆RuvCIII-2 ∆HNH ∆REC2 4QC_HNHpK Sp-dCas9

dCas9 is a catalytically inactive Cas9, which still retains it's ability to bind to DNA. This truncated Sp-dCas9 has been improved upon to increase DNA binding affinity. It can be fused to an effector protein to achieve targetting to specific locus. This is an improvement from part Part:BBa_K2316001

Usage and Biology

It was previously shown that SpdCas9 can be truncated and improved by mutation of DNA backbone proximal amino acids. This part is a further improvement upon Part:BBa_K2316001, where additional mutation and site specific fusion of a cationic peptide demonstrated further improvement upon the truncated dCas9. <p>Improving upon previously submitted part ∆RuvCIII-2 ∆HNH ∆REC2 Sp-dCas9 Enhanced (BBa_K2316001), also referred to as ∆3ple 5_6_10, mutation at the position 15 is found to produce the best performing variant. Further compounding of mutations appear to no improve ∆3ple 5_6_10_15. These mutations are indicated in short form on name of this part as “4QC”.

Sequence and Features