Difference between revisions of "Part:BBa K2570019"
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[[File:FileT--FJNU-China--TET-OFF_1.png|600px|thumb|center|Fig. (A) Conceptual diagram of metabolic state switching from the cell growth mode to suicide mode. (B) Design of the synthetic metabolic regulator.]]
 
[[File:FileT--FJNU-China--TET-OFF_1.png|600px|thumb|center|Fig. (A) Conceptual diagram of metabolic state switching from the cell growth mode to suicide mode. (B) Design of the synthetic metabolic regulator.]]
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(A)Conceptual diagram of metabolic state switching from the cell growth mode to suicide mode.
 
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(B)Design of the synthetic metabolic regulator circuit.
 
  
 
AraC repressor inhibits transcription from the PBAD promoter. When transcription by the PBAD promoter is induced with arabinose addition, expression of tetR and GFP is promoted and the expression of mCherry under the PLtetO1 promoter is inhibited by the TetR repressor. Without arabinose addition, expression of tetR and GFP under the PBAD promoter is inhibited by the araC repressor and the expression of mCherry under the PLtetO1 promoter is promoted.
 
AraC repressor inhibits transcription from the PBAD promoter. When transcription by the PBAD promoter is induced with arabinose addition, expression of tetR and GFP is promoted and the expression of mCherry under the PLtetO1 promoter is inhibited by the TetR repressor. Without arabinose addition, expression of tetR and GFP under the PBAD promoter is inhibited by the araC repressor and the expression of mCherry under the PLtetO1 promoter is promoted.

Revision as of 09:02, 15 October 2018


tetR+GFP+mazF+PLtetO1+RFP

The Tet-Off system which turns expression of its controlling genes OFF by the addition of tetracycline and its derivative is often used for this purpose and has been applied to several organisms.

The figure shows a strategy for metabolic state switching module using a synthetic metabolic regulator.

Fig. (A) Conceptual diagram of metabolic state switching from the cell growth mode to suicide mode. (B) Design of the synthetic metabolic regulator.

AraC repressor inhibits transcription from the PBAD promoter. When transcription by the PBAD promoter is induced with arabinose addition, expression of tetR and GFP is promoted and the expression of mCherry under the PLtetO1 promoter is inhibited by the TetR repressor. Without arabinose addition, expression of tetR and GFP under the PBAD promoter is inhibited by the araC repressor and the expression of mCherry under the PLtetO1 promoter is promoted.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2590
    Illegal AgeI site found at 2702
  • 1000
    COMPATIBLE WITH RFC[1000]