Difference between revisions of "Part:BBa K2680124"
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<partinfo>BBa_K2680124 short</partinfo>
 
<partinfo>BBa_K2680124 short</partinfo>
  
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pTlpAr promoter in a 3G-compatible form. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].  
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pTlpAr flanked by prefix sticky end A and suffix sticky end B in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].  
  
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pTlpAr is the reverse complement of pTlpA (<partinfo>BBa_K2680123</partinfo>). Transcribes 200-fold weaker than pTlpA. Flanked by prefix sticky end A and suffix sticky end B.
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pTlpAr is the reverse complement of the heat-sensitive promoter [https://parts.igem.org/Part:BBa_K2500003 pTlpA]. Transcribes 200-fold weaker than pTlpA. Flanked by prefix sticky end A and suffix sticky end B.
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 22:03, 14 October 2018


3G pTlpAr

pTlpAr flanked by prefix sticky end A and suffix sticky end B in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].

pTlpAr is the reverse complement of the heat-sensitive promoter pTlpA. Transcribes 200-fold weaker than pTlpA. Flanked by prefix sticky end A and suffix sticky end B.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 41
    Illegal BsaI.rc site found at 109