Difference between revisions of "Part:BBa K2886007"

Revision as of 19:10, 14 October 2018

VEGF-His9

We present VEGF-His9 as our best basic part to enable iGEMers to express and purify VEGFA-165 easily.

VEGF (vascular endothelial growth factor), originally known as VPF (vascular permeability factor), is a ligand commonly used in research. As a kind of endogenous growth factor produced by cells humans and mice, VEGF has various biological effects such as stimulates the formation of blood vessels and enhance the permeability of blood vessels. VEGF is a big family, and there are a lot of types of VEGF including VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E and PGF, of which VEGF-121 and VEGF-165 are two main types in body and research.

VEGF-121 was introduced to iGEM by Slovenia in 2012 (BBa_K782061) and then was improved by SYSU-CHINA in 2017 (BBa_K2298l002). VEGF-165 was also firstly introduced to iGEM by NYMU_Taiwan in 2012(BBa_K896922). However, there is no detail in the part wiki at all, and we actually find this part using registry blast. This year, Fudan-CHINA makes new improvements to VEGF-165 to make it more useful and accessible to enrich the choices of iGEMers.

We perfected the description of VEGF-165 to make more iGEMers can know about and search for VEGF-165 expediently. To test our STEP system, we need a large amount of VEGF-165. However, buying VEGF from companies will be really expensive. The average price of 1mg VEGF-165 is as high as 10,000 yuan (nearly 5000 dollars). Thus, we decide to construct plasmid and express VEGF-165 on ourselves. We designed a sequence coding VEGF-165 followed by a 9×His tag and then entrusted Sangon Biotech to synthesis it totally on the backbone Vesalius (talk) 19:10, 14 October 2018 (UTC). To our delighted, we succeeded in expressing VEGF-165 in E. coli and then purifying it. Here are the result.;

From the gel, we can see that19:10, 14 October 2018 (UTC)19:10, 14 October 2018 (UTC)19:10, 14 October 2018 (UTC)19:10, 14 October 2018 (UTC)

Considering the successful results, we are willing to share this part with all iGEMer around the world to express and purify VEGF-165 on yourselves at an extremely low cost, enabling you to spare more funds for other experiments and human practice.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 166

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 <partinfo>BBa_K2886007 short</partinfo>
-VEGF (vascular endothelial growth factor) is a common ligand used in synthetic biology. And there are a lot of types of VEGF in human and rice like VEGF-121, VEGF-165 and so on. And in human, there are two main types of VEGFA: VEGF-121 and VEGF-165.
+We present VEGF-His9 as our best basic part to enable iGEMers to express and purify VEGFA-165
+easily.
-VEGF-121 was firstly introduced to iGEM by  iGEM12_Slovenia in 2012, which was from mouse. In 2017, iGEM17_SYSU-CHINA improved this part by isolating it from the initial composite part and submitting the counterpart in human, making VEGF-121 more mobile and suitable for human cell lines.
+VEGF (vascular endothelial growth factor), originally known as VPF (vascular permeability factor), is a ligand commonly used in research. As a kind of endogenous growth factor produced by cells humans and mice, VEGF has various biological effects such as stimulates the formation of blood vessels and enhance the permeability of blood vessels. VEGF is a big family, and there are a lot of types of VEGF including VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E and PGF, of which VEGF-121 and VEGF-165 are two main types in body and research.
-VEGF-165 also was firstly introduced to iGEM by  iGEM12_NYMU_Taiwan in 2012(BBa_K896922). However, there is no detail in the part wiki at all, and we actually find this part through blast tools. This year, Fudan-CHINA makes new improvements to VEGFA to make it more useful  and enrich the chioces of iGEMers.
+VEGF-121 was introduced to iGEM by Slovenia in 2012 (BBa_K782061) and then was improved by SYSU-CHINA in 2017 (BBa_K2298l002). VEGF-165 was also firstly introduced to iGEM by NYMU_Taiwan in 2012(BBa_K896922). However, there is no detail in the part wiki at all, and we actually find this part using registry blast. This year, Fudan-CHINA makes new improvements to VEGF-165 to make it more useful and accessible to enrich the choices of iGEMers.
-According to literatures, VEGF-165 has a higher affinity to heparin and might has higher activity than VEGF-121. Besides, VEGF-165 is much more adopted in research compared with VEGF-121. And what's more, buying VEGF-165 from companies might be very expensive, so we add a His tag that could help us to express and purify VEGF-165 easily. And We did succeed in collecting purified VEGF-165 with the part we constructed, proving its reliability. What is also worth mentioning is that we could also us GS tag, but given it's even longer than VEGF itself, we turned to His tag ultimately.
+We perfected the description of VEGF-165 to make more iGEMers can know about and search for VEGF-165 expediently. To test our STEP system, we need a large amount of VEGF-165. However, buying VEGF from companies will be really expensive. The average price of 1mg VEGF-165 is as high as 10,000 yuan (nearly 5000 dollars). Thus, we decide to construct plasmid and express VEGF-165 on ourselves. We designed a sequence coding VEGF-165 followed by a 9×His tag and then entrusted Sangon Biotech to synthesis it totally on the backbone [[User:Vesalius|Vesalius]] ([[User talk:Vesalius|talk]]) 19:10, 14 October 2018 (UTC). To our delighted, we succeeded in expressing VEGF-165 in E. coli and then purifying it. Here are the result.;
+From the gel, we can see that19:10, 14 October 2018 (UTC)19:10, 14 October 2018 (UTC)19:10, 14 October 2018 (UTC)19:10, 14 October 2018 (UTC)
+Considering the successful results, we are willing to share this part with all iGEMer around the world to express and purify VEGF-165 on yourselves at an extremely low cost, enabling you to spare more funds for other experiments and human practice.
-All in all, through our work, VEGF becomes much more available and easy to use.
 <!-- Add more about the biology of this part here