Difference between revisions of "Part:BBa K2729010"
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==reference== | ==reference== | ||
| − | - [https://pubs.acs.org/doi/10.1021/bi500724k Nemésio H, Villalaín J. Membranotropic regions of the dengue virus prM | + | - [https://pubs.acs.org/doi/10.1021/bi500724k Nemésio H, Villalaín J. Membranotropic regions of the dengue virus prM protein. Biochemistry. 2014 Aug 19;53(32):5280-9. doi: 10.1021/bi500724k. Epub 2014 Aug 6. PubMed PMID: 25076193.] |
| − | protein. Biochemistry. 2014 Aug 19;53(32):5280-9. doi: 10.1021/bi500724k. Epub | + | |
| − | 2014 Aug 6. PubMed PMID: 25076193. | + | |
| − | ] | + | |
Revision as of 14:31, 14 October 2018
DENV1 ancC-prM-E (Dengue Virus Serotype I)
Flavivirus Capsid (C), prM (precursor Membrane) and Envelope (E) are structural proteins responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 1831
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 1831
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1245
Illegal BglII site found at 1856 - 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 1831
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 1831
Illegal AgeI site found at 1450 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 217
Illegal BsaI.rc site found at 367
Characterization
The prM protein consists of an N-terminal pr domain followed by the M protein separated by a furin cleavage site.(15) The pr part of the protein consists mainly of β-strands, whereas the M portion consists of a linear structure followed by a mainly α-helical stem region and two transmembrane helices.