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Revision as of 13:43, 14 October 2018


Legionella Sensing LacI Repressing Alpha Toxicity Experimental Device

GFP is expressed with the gRNA to test toxicity




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1077
    Illegal NgoMIV site found at 1106
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 856