Difference between revisions of "Part:BBa K2841500"

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GFP is expressed with the gRNA to test toxicity
 
GFP is expressed with the gRNA to test toxicity
  
https://static.igem.org/mediawiki/parts/thumb/4/4c/T--Warwick--2018-IPTG-Alpha-Construct-Induction-Graph.png/800px-T--Warwick--2018-IPTG-Alpha-Construct-Induction-Graph.png
 
https://static.igem.org/mediawiki/parts/thumb/3/31/T--Warwick--2018-IPTG-Alpha-Construct-Induction-Graph-OD600.png/800px-T--Warwick--2018-IPTG-Alpha-Construct-Induction-Graph-OD600.png
 
https://static.igem.org/mediawiki/parts/thumb/f/f0/T--Warwick--2018-IPTG-Alpha-Construct-Induction-Graph-Raw-Flourescence.png/800px-T--Warwick--2018-IPTG-Alpha-Construct-Induction-Graph-Raw-Flourescence.png
 
  
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[[Image:https://static.igem.org/mediawiki/parts/4/4c/T--Warwick--2018-IPTG-Alpha-Construct-Induction-Graph.png|400px|right|Characterization of popular BioBrick RBSs]]
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https://static.igem.org/mediawiki/parts/3/31/T--Warwick--2018-IPTG-Alpha-Construct-Induction-Graph-OD600.png
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https://static.igem.org/mediawiki/parts/f/f0/T--Warwick--2018-IPTG-Alpha-Construct-Induction-Graph-Raw-Flourescence.png
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 13:42, 14 October 2018


Legionella Sensing LacI Repressing Alpha Toxicity Experimental Device

GFP is expressed with the gRNA to test toxicity



T--Warwick--2018-IPTG-Alpha-Construct-Induction-Graph-OD600.png T--Warwick--2018-IPTG-Alpha-Construct-Induction-Graph-Raw-Flourescence.png Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1077
    Illegal NgoMIV site found at 1106
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 856