Difference between revisions of "Part:BBa K2610025"
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iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry. | iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry. | ||
| − | We fused promoters to green fluorescent protein and stressed transformed | + | We fused promoters to green fluorescent protein and stressed transformed DH5α cells for several hours. |
[[File:T--Leiden--heatmap.png|750px]] | [[File:T--Leiden--heatmap.png|750px]] | ||
| Line 14: | Line 14: | ||
<span style="font-size:1em"><b>Figure 1.</b> Heatmap with stress-activated promoters </span> | <span style="font-size:1em"><b>Figure 1.</b> Heatmap with stress-activated promoters </span> | ||
| − | As can be observed in Figure 1 the | + | As can be observed in Figure 1 the Spy promoter is activated by nalidixic acid, ethanol and chloramphenicol. This is indicative of a similar mechanism of actions for these compounds. |
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Latest revision as of 12:59, 14 October 2018
pSpy
Spy, an ATP-independent chaperone, is highly upregulated in the presence of protein unfolding agent ethanol. It decreases protein degradation.
Usage and Biology
iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry. We fused promoters to green fluorescent protein and stressed transformed DH5α cells for several hours.
Figure 1. Heatmap with stress-activated promoters
As can be observed in Figure 1 the Spy promoter is activated by nalidixic acid, ethanol and chloramphenicol. This is indicative of a similar mechanism of actions for these compounds.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]