Difference between revisions of "Part:BBa K2762004:Design"

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===Design Notes===
 
===Design Notes===
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The whole genome sequence of Synechococcus sp. PCC7002 is found in NCBI. We first codon optimized the CA sequence and sent it to IDT for gene synthesis.  
+
The whole genome sequence of <i>Synechococcus sp.</i> PCC7002 is found in NCBI. We first codon optimized the CA sequence and sent it to IDT for gene synthesis.  
 
The gene fragment is amplified via PCR reaction for cloning.
 
The gene fragment is amplified via PCR reaction for cloning.
  

Revision as of 12:47, 14 October 2018


CcaA (formerly icfA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The whole genome sequence of Synechococcus sp. PCC7002 is found in NCBI. We first codon optimized the CA sequence and sent it to IDT for gene synthesis. The gene fragment is amplified via PCR reaction for cloning.

Source

Synechococcus elongatus PCC7002

https://www.ncbi.nlm.nih.gov/nuccore/169884305

References