Difference between revisions of "Part:BBa K2547003"
Line 3: | Line 3: | ||
<partinfo>BBa_K2547003 short</partinfo> | <partinfo>BBa_K2547003 short</partinfo> | ||
− | + | This is an improved part of carbonic anhydrase (csoS3) from Halothiobacillus neapolitanus which converts the incoming bicarbonate into carbon dioxide in the carboxysome, a step that is essential for CO2 fixation. We have optimized codon sequence in the coding sequence (CDS) to make the carbonic anhydrase gene more suitable for expression in E. coli. In addition to codon optimization, we have added a His-tag to the C-terminal of the CDS that is used for purification of carbonic anhydrase protein. | |
Revision as of 10:53, 14 October 2018
Carbonic anhydrase (csoS3)-His
This is an improved part of carbonic anhydrase (csoS3) from Halothiobacillus neapolitanus which converts the incoming bicarbonate into carbon dioxide in the carboxysome, a step that is essential for CO2 fixation. We have optimized codon sequence in the coding sequence (CDS) to make the carbonic anhydrase gene more suitable for expression in E. coli. In addition to codon optimization, we have added a His-tag to the C-terminal of the CDS that is used for purification of carbonic anhydrase protein.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
The coding sequence of Carbonic anhydrase csoS3 from original part was codon-optimized, and also a His tag was added to the end, to ensure that Carbonic anhydrase csoS3 could be expressed in E. coli BL21 (DE3) and retained potent carbonic anhydrase activity (Fig. 1).
First, the original coding sequence of csoS3 and the coding sequence with codon optimization were synthesized, and cloned into the pET-30a (+) expression vectors, respectively. The correctness of the two recombinant plasmids was verified by PCR (Fig. 2).
Subsequently, the expression of two csoS3 plasmids in E. coli was detected via SDS-PAGE and Coomassie blue staining. As shown in Fig. 3, the result presented that the expression of csoS3 original part in E. coli was relatively low, and the expression of codon-optimized csoS3 new part in E. coli was higher than original part.
To further demonstrate the activity of our new part, new part of csoS3 carbonic anhydrase was purified through Ni-chelating affinity chromatography and detected by SDS-PAGE and Coomassie blue staining, as shown in Fig. 4. Then, the activity of csoS3 was measured via esterase method, and the enzyme activity was about 22.84 U/mL.
In conclusion, our results demonstrated that the function of csoS3 new part has been improved with higher expression and activity than original part.