Difference between revisions of "Part:BBa K2675195:Design"

 
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===Design Notes===
 
===Design Notes===
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BBa_J23110 BBa_K2675071 BBa_K2675095 BBa_K2675016 BBa_K2675006 BBa_K2675030
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[[Part:BBa_J23110|BBa_J23110]] constitutive promoter + pAimX(full-noTerminator-v6) promoter ([[Part:BBa_K2675095|BBa_K2675095]]) + custom made RBS ([[Part:BBa_K2675016|BBa_K2675016]]) + sfGFP-LVAtag coding sequence ([[Part:BBa_K2675006|BBa_K2675006]]) + the strong L3S2P56 Terminator ([[Part:BBa_K2675030|BBa_K2675030]])
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+
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===Source===
 
===Source===
  
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DNA synthesis & Assembly
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+
  
 
===References===
 
===References===
 +
[1] Erez Z, Steinberger-Levy I, Shamir M, Doron S, Stokar-Avihail A, Peleg Y, Melamed S, Leavitt A, Savidor A, Albeck S, Amitai G, Sorek R. Communication between viruses guides lysis-lysogeny decisions. Nature (2017) 541, 488-493.

Revision as of 08:55, 14 October 2018


sfGFP-LVAtag expression cassette under the control of J23110 and pAimX(full-noTerminator-v6) promote


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 80
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 80
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 80
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 80
    Illegal AgeI site found at 227
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_J23110 constitutive promoter + pAimX(full-noTerminator-v6) promoter (BBa_K2675095) + custom made RBS (BBa_K2675016) + sfGFP-LVAtag coding sequence (BBa_K2675006) + the strong L3S2P56 Terminator (BBa_K2675030)

Source

DNA synthesis & Assembly

References

[1] Erez Z, Steinberger-Levy I, Shamir M, Doron S, Stokar-Avihail A, Peleg Y, Melamed S, Leavitt A, Savidor A, Albeck S, Amitai G, Sorek R. Communication between viruses guides lysis-lysogeny decisions. Nature (2017) 541, 488-493.