Difference between revisions of "Part:BBa K2708009"

Latest revision as of 07:01, 14 October 2018

Promoter

This biobrick is an improvment of the biobrick BBa_I14018 designed by Group: Antiquity(2004)

Characterizing Part:

We modified the sequence of the promoter BBa_I14018, and got the promoter BBa_K2708009. Then we assembled these two promoter with reporter BBa_E0840 and transforming plasmid to Ecoli DH5α, measuring green fluorescence to characterize the effect of the promoter.

The figure below is the fluorescence results we measured in the eighth hour of culture. Excitation and emission are 480nm and 520nm, E0840 is the negative control. The results show that the promoter K2708009 has a stronger starting ability than I14018.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 13: / Line 13: @@
 [[Image: T--BIT--Figure_Improve2.png |center|]]
 <p >We modified the sequence of the promoter BBa_I14018, and got the promoter BBa_K2708009.
-Then we assembled these two promoter with repoter BBa_E0840 and transforming plasmid to Ecoli DH5α, measuring green fluorescence to characterize the effect of the promoter.</p>
+Then we assembled these two promoter with reporter BBa_E0840 and transforming plasmid to Ecoli DH5α, measuring green fluorescence to characterize the effect of the promoter.</p>
 [[Image: T--BIT--Figure_Improve1.png |center|]]
 The figure below is the fluorescence results we measured in the eighth hour of culture. Excitation and emission are 480nm and 520nm, E0840 is the negative control. The results show that the promoter K2708009 has a stronger starting ability than I14018.