Difference between revisions of "Part:BBa K2637027:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | TDH3 Promoter + AD + KaiB + ADH1 Terminator. And KaiB has been optimized in yeast. | |
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===Source=== | ===Source=== | ||
Latest revision as of 05:29, 14 October 2018
AD-KaiB cassette with TDH3 Promoter and ADH1 Terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 366
Illegal BglII site found at 1633 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
TDH3 Promoter + AD + KaiB + ADH1 Terminator. And KaiB has been optimized in yeast.
Source
TDH3P and ADH1T are the promoter and terminator of yeast. Gal4 activation domain is from yeast genome and KaiB is from Synechococcus elongatus PCC7942.