Difference between revisions of "Part:BBa K2637026:Design"

(→‎Source)
(→‎Source)
Line 10: Line 10:
  
 
===Source===
 
===Source===
−
TDH3P and ADH1T are the promoter and terminator of yeast. Gal4 DNA binding domain is from yeast genome and CikA is from Synechococcus elongatus PCC7942.
+
TEF1P and TEF1T are the promoter and terminator of yeast. Gal4 DNA binding domain is from yeast genome and CikA is from Synechococcus elongatus PCC7942.
  
 
===References===
 
===References===

Revision as of 05:28, 14 October 2018


BD-CikA cassette with TEF1 Promoter and TEF1 Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1109
    Illegal BamHI site found at 3169
    Illegal XhoI site found at 753
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 672
    Illegal BsaI.rc site found at 266


Design Notes

TDH3 Promoter + BD + CikA + ADH1 Terminator. And CikA has been optimized in yeast.

Source

TEF1P and TEF1T are the promoter and terminator of yeast. Gal4 DNA binding domain is from yeast genome and CikA is from Synechococcus elongatus PCC7942.

References