Difference between revisions of "Part:BBa K2637026:Design"
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===Source=== | ===Source=== | ||
| − | + | TDH3P and ADH1T are the promoter and terminator of yeast. Gal4 DNA binding domain is from yeast genome and CikA is from Synechococcus elongatus PCC7942. | |
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===References=== | ===References=== | ||
Revision as of 05:26, 14 October 2018
BD-CikA cassette with TEF1 Promoter and TEF1 Terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1109
Illegal BamHI site found at 3169
Illegal XhoI site found at 753 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 672
Illegal BsaI.rc site found at 266
Design Notes
TDH3 Promoter + BD + CikA + ADH1 Terminator. And CikA has been optimized in yeast.
Source
TDH3P and ADH1T are the promoter and terminator of yeast. Gal4 DNA binding domain is from yeast genome and CikA is from Synechococcus elongatus PCC7942.