Difference between revisions of "Part:BBa K2637026:Design"

 
(Design Notes)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
645
+
TDH3 Promoter + BD + CikA + ADH1 Terminator. And CikA has been optimized in yeast.
 
+
 
+
  
 
===Source===
 
===Source===

Revision as of 05:26, 14 October 2018


BD-CikA cassette with TEF1 Promoter and TEF1 Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1109
    Illegal BamHI site found at 3169
    Illegal XhoI site found at 753
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 672
    Illegal BsaI.rc site found at 266


Design Notes

TDH3 Promoter + BD + CikA + ADH1 Terminator. And CikA has been optimized in yeast.

Source

46456

References