Difference between revisions of "Part:BBa K2637025:Design"
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===Source=== | ===Source=== | ||
| − | + | TDH3P and ADH1T are the promoter and terminator of yeast. Gal4 DNA binding domain is from yeast genome and SasA is from Synechococcus elongatus PCC7942. | |
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===References=== | ===References=== | ||
Latest revision as of 05:19, 14 October 2018
BD-SasA cassette with TDH3 Promoter and ADH1 Terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 366
Illegal BglII site found at 2058
Illegal BglII site found at 2127
Illegal XhoI site found at 1291 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1210
Design Notes
TDH3 Promoter + BD + SasA + ADH1 Terminator. And SasA has been optimized in yeast.
Source
TDH3P and ADH1T are the promoter and terminator of yeast. Gal4 DNA binding domain is from yeast genome and SasA is from Synechococcus elongatus PCC7942.