Difference between revisions of "Part:BBa K2637039"
| Line 3: | Line 3: | ||
<partinfo>BBa_K2637039 short</partinfo> | <partinfo>BBa_K2637039 short</partinfo> | ||
| − | We constructed this pRS416 plasmid carrying the genes of mCherrry with the Gal2 promoter and ADH1T terminator by harnessing the principles of yeast homologous recombination. This cassette which played a prominent role in our measurement process | + | We constructed this pRS416 plasmid carrying the genes of mCherrry with the Gal2 promoter and ADH1T terminator by harnessing the principles of yeast homologous recombination. This cassette which played a prominent role in our measurement process would be a segment of our parallel experiments aimed to minimize the false positive phenomenon of the yeast two-hybrid system. We selected the mCherry fluorescent protein with the help of our team members belonging to the modeling group since we take priority of its brief latency time and quick degradation rate. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 05:05, 14 October 2018
Gal2 promoter+mCherry+ADH1 terminator
We constructed this pRS416 plasmid carrying the genes of mCherrry with the Gal2 promoter and ADH1T terminator by harnessing the principles of yeast homologous recombination. This cassette which played a prominent role in our measurement process would be a segment of our parallel experiments aimed to minimize the false positive phenomenon of the yeast two-hybrid system. We selected the mCherry fluorescent protein with the help of our team members belonging to the modeling group since we take priority of its brief latency time and quick degradation rate. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 529