Difference between revisions of "Part:BBa K2637039"

Revision as of 05:01, 14 October 2018

Gal2 promoter+mCherry+ADH1 terminator

We constructed this pRS416 plasmid carrying the genes of mCherrry with the Gal2 promoter and ADH1T terminator by harnessing the principles of yeast homologous recombination. This cassette which played a prominent role in our measurement process to be a segment of our parallel experiments aimed to minimize the false positive phenomenon of the yeast two-hybrid system. We selected the mCherry fluorescent protein with the help of our team members belonging to the modeling group since we take priority of its brief latency time and quick degradation rate. Sequence and Features

Assembly Compatibility:

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COMPATIBLE WITH RFC[10]
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COMPATIBLE WITH RFC[12]
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COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
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INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 529

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 <partinfo>BBa_K2637039 short</partinfo>
-We constructed this pRS416 plasmid carrying the genes of mCherrry with the Gal2 promoter and ADH1T terminator by harnessing the principles of yeast homologous recombination. And only if the reporter genes function normally can we ensure that the system we reconstructed in the Saccharomyces cerevisiae succeeds.
+We constructed this pRS416 plasmid carrying the genes of mCherrry with the Gal2 promoter and ADH1T terminator by harnessing the principles of yeast homologous recombination. This cassette which played a prominent role in our measurement process to be a segment of our parallel experiments aimed to minimize the false positive phenomenon of the yeast two-hybrid system. We selected the mCherry fluorescent protein with the help of our team members belonging to the modeling group since we take priority of its brief latency time and quick degradation rate.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===