Difference between revisions of "Part:BBa K2637038"

 
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<partinfo>BBa_K2637038 short</partinfo>
 
<partinfo>BBa_K2637038 short</partinfo>
  
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The cassatte is composed of Gal1 promoter, NanoLuc and ADH1 terminator. In Y2H system it can be used to characterize the fusion proteins.
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We constructed this cassette which played a prominent role in our measurement process to be a segment of our parallel experiments aimed to minimize the false positive phenomenon of the yeast two-hybrid system. We chose the NanoLuc from the part since it would produce high intensity, glow-type luminescence. Moreover, it also possesses a number of physical properties that make it an excellent reporter protein: small, monomeric enzyme, high thermal stability and so on.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 04:59, 14 October 2018


Gal1 promoter+NanoLuc+ADH1 terminator

We constructed this cassette which played a prominent role in our measurement process to be a segment of our parallel experiments aimed to minimize the false positive phenomenon of the yeast two-hybrid system. We chose the NanoLuc from the part since it would produce high intensity, glow-type luminescence. Moreover, it also possesses a number of physical properties that make it an excellent reporter protein: small, monomeric enzyme, high thermal stability and so on.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]