Difference between revisions of "Part:BBa K2637037"

 
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<partinfo>BBa_K2637037 short</partinfo>
 
<partinfo>BBa_K2637037 short</partinfo>
  
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The cassatte is composed of Gal1 promoter, EYFP and ADH1 terminator. In Y2H system it can be used to characterize the fusion proteins.
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We constructed this cassette which played a prominent role in our measurement process to be a segment of our parallel experiments aimed to minimize the false positive phenomenon of the yeast two-hybrid system. We selected the EYFP fluorescent protein with the help of our team members belonging to the modeling group since we take priority of its brief latency time and quick degradation rate.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 04:57, 14 October 2018


Gal1 promoter+EYFP+ADH1T

We constructed this cassette which played a prominent role in our measurement process to be a segment of our parallel experiments aimed to minimize the false positive phenomenon of the yeast two-hybrid system. We selected the EYFP fluorescent protein with the help of our team members belonging to the modeling group since we take priority of its brief latency time and quick degradation rate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1125