Difference between revisions of "Part:BBa K2623025"

Latest revision as of 03:53, 14 October 2018

Archaeal ribosomal protein protein L7Ae fused with SpyCatcher and mRFP1.

Archaeal ribosomal protein protein L7Ae owns the ability to bind with RNA with C/Dbox RNA structure. Through the isopeptide bonds formed between SpyTag and SpyCatcher, L7Ae can be anchored with OmpA, and then secreted into OMVs. To transport our L7Ae into the periplasmic space, we insert TorA leader sequence to the circuitry and conjugate mRFP1 with L7Ae to validate the SpyTag/SpyCatcher bioconjugation.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1168
Illegal NheI site found at 1191
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 2325
Illegal AgeI site found at 2437
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2623025 short</partinfo>
-Archaeal ribosomal protein protein L7Ae owns the ability to bind with RNA with C/Dbox RNA structure. Through the isopeptide bonds formed between SpyTag and SpyCatcher, L7Ae can be anchored with OmpA, and then secreted into OMVs. To transport our L7Ae into the periplasmic space, we inserted TorA leader sequence to the circuitry. mRFP1 is conjugated with L7Ae to validate the SpyTag/SpyCatcher bioconjugation.
+Archaeal ribosomal protein protein L7Ae owns the ability to bind with RNA with C/Dbox RNA structure. Through the isopeptide bonds formed between SpyTag and SpyCatcher, L7Ae can be anchored with OmpA, and then secreted into OMVs. To transport our L7Ae into the periplasmic space, we insert TorA leader sequence to the circuitry and conjugate mRFP1 with L7Ae to validate the SpyTag/SpyCatcher bioconjugation.
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