Difference between revisions of "Part:BBa K2675095:Design"
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
pAimX(full) promoter of phage phi3T ([[Part:BBa_K2675020|BBa_K2675020]]) with mutations in the putative terminator.
+
pAimX(full) promoter of phage phi3T ([[Part:BBa_K2675020|BBa_K2675020]]) with mutations in the putative terminator. These mutations disrupted also the pAimX(2) putative promoter.
  
 
===Source===
 
===Source===

Revision as of 20:51, 13 October 2018


pAimX(full-noTerminator-v6) promoter of phage phi3T


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 40
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 40
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 40
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 40
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pAimX(full) promoter of phage phi3T (BBa_K2675020) with mutations in the putative terminator. These mutations disrupted also the pAimX(2) putative promoter.

Source

selection from a random sequence library derived by PCR with degenerate primers on BBa_K2675020

References

[1] Erez Z, Steinberger-Levy I, Shamir M, Doron S, Stokar-Avihail A, Peleg Y, Melamed S, Leavitt A, Savidor A, Albeck S, Amitai G, Sorek R. Communication between viruses guides lysis-lysogeny decisions. Nature (2017) 541, 488-493.