Difference between revisions of "Part:BBa K2680123"
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<partinfo>BBa_K2680123 short</partinfo> | <partinfo>BBa_K2680123 short</partinfo> | ||
− | 3G-compatible pTlpA promoter <partinfo>BBa_K2500003</partinfo>. Repressed by | + | 3G-compatible pTlpA promoter <partinfo>BBa_K2500003</partinfo>. Repressed by TlpA, a heat-sensitive protein which begins to deactivate at 37C. pTlpA also transcribes 500 fold weaker in the reverse direction. Flanked by prefix sticky end A and suffix sticky end B. |
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Revision as of 19:43, 13 October 2018
3G pTlpA
3G-compatible pTlpA promoter BBa_K2500003. Repressed by TlpA, a heat-sensitive protein which begins to deactivate at 37C. pTlpA also transcribes 500 fold weaker in the reverse direction. Flanked by prefix sticky end A and suffix sticky end B.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 41
Illegal BsaI.rc site found at 109