Difference between revisions of "Part:BBa K2841510"
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The protein is shown in wheat colour <bbpart>BBa_K2841510</bbpart>. The RNA cis acting factor (for the dCAS9 protein to recognise the gRNA) is in grey <bbpart>BBa_K2841514</bbpart>. The gRNA's targeting region (what specifies the target sequence) is in green <bbpart>BBa_K2841514</bbpart>.
 
  
 
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Revision as of 18:49, 13 October 2018


Streptococcus Pyogenes dCAS9

The dCAS9 protein used in the CRISPR repressor 2013 paper. The sequence of the part has been modified to be compatible with iGEM standard. The dCAS9 accepts a gRNA (BBa_K2841514) which directs it to a site of interest. The structure for the protein family has been solved.

T--Warwick--2018-4zt9.gif

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]