Difference between revisions of "Part:BBa K2865006"

Revision as of 16:16, 13 October 2018

CMV promoter-eGFP-poly(A)

This part contains CMV promoter, eGFP reporter in RFC25 format(BBa_K2865005), and SV40 polyA terminator (BBa_K2865004). This part is constructed as a control with our part BNP promoter-eGFP-poly(A) (BBa_K2865008).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1430
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K2865006 short</partinfo>
+<p class="MsoNormal"><span lang="EN-US">
-This part is a generator of eGFP in mammalian cells. It is constructed as a control with our part BBa_K2865008, which is composed of BNP promoter, eGFP, and ployA.
+This part contains CMV promoter, eGFP reporter in RFC25 format(BBa_K2865005), and SV40 polyA terminator (BBa_K2865004). This part is constructed as a control with our part BNP promoter-eGFP-poly(A) (BBa_K2865008). </span></p>
 <!-- Add more about the biology of this part here
-===Usage and Biology===
+===Experiment===
+<B>Fluorescence microscope</B>
+<p class="MsoNormal"><span lang="EN-US">We transfected H9C2 cell with target plasmids, BBa_K2865006 and BBa_K2865005, via liposome transfection. After 48 hours’ expression, we detected the cells using fluorescence microscope. And we have taken clearly photos. Wo all know that the CMV promoter is widely and commonly used in plasmids or viruses designed for gene expression in mammalian cells since it is a very strong promoter and drives constitutive expression of genes under its control. And we have to admit that the transfection efficiency is low. </span></p>
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>