Difference between revisions of "Part:BBa K2623027"

 
 
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<partinfo>BBa_K2623027 short</partinfo>
 
<partinfo>BBa_K2623027 short</partinfo>
  
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Under the induction of Arabinose, this part can code siRNA with C/Dbox RNA structure and then bind with L7Ae protein to be encapsulated into OMVs.
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===Summary===
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Under the induction of Arabinose, this part can code siRNA with C/Dbox RNA structure target for KrasG12D in human pancreatic ductal adenocarcinoma (PDAC) [1]. With the isopeptide bond formed between SpyTag and SpyCatcher, and the ability of L7Ae to be bind with C/Dbox, we can produce customizable and cell-free OMVs containing specific siRNA to target for oncogenic gene.
  
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<br><table><tr><th>
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[[Image:SiRNA.png|thumb|420px|Genetic circuit of the Box part.]]</th><th></table>
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===References===
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[1] Kamerkar S, Lebleu V S, Sugimoto H, et al. Exosomes facilitate therapeutic targeting of oncogenic KRAS in pancreatic cancer[J]. Nature, 2017, 546(7659):498-503.
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 14:57, 13 October 2018


siRNA with C/Dbox RNA structure to bind with L7Ae (BBa_K2623026).

Summary

Under the induction of Arabinose, this part can code siRNA with C/Dbox RNA structure target for KrasG12D in human pancreatic ductal adenocarcinoma (PDAC) [1]. With the isopeptide bond formed between SpyTag and SpyCatcher, and the ability of L7Ae to be bind with C/Dbox, we can produce customizable and cell-free OMVs containing specific siRNA to target for oncogenic gene.


Genetic circuit of the Box part.

References

[1] Kamerkar S, Lebleu V S, Sugimoto H, et al. Exosomes facilitate therapeutic targeting of oncogenic KRAS in pancreatic cancer[J]. Nature, 2017, 546(7659):498-503. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1268
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1298
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1304
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961