Difference between revisions of "Part:BBa K2669000:Experience"
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After transforming our cells with a low copy amplicilin plasmid containing this composite part, cell lysis and affinity chromotography were used to extract UnaG from our cells. <b>Please note:</b> The exact procedure can be found at the end of <a href="http://2018.igem.org/wiki/index.php?title=Team:Uppsala/UnaG"><b>our UnaG wiki page</b></a>. Conducting "bilirubin tests" (the addition of a small amount of bilirubin dissolved in chloroform to samples) allowed us to see if UnaG was present in our samples, since as mentioned earlier UnaG fluoresces in the presence of bilirubin. </p> | After transforming our cells with a low copy amplicilin plasmid containing this composite part, cell lysis and affinity chromotography were used to extract UnaG from our cells. <b>Please note:</b> The exact procedure can be found at the end of <a href="http://2018.igem.org/wiki/index.php?title=Team:Uppsala/UnaG"><b>our UnaG wiki page</b></a>. Conducting "bilirubin tests" (the addition of a small amount of bilirubin dissolved in chloroform to samples) allowed us to see if UnaG was present in our samples, since as mentioned earlier UnaG fluoresces in the presence of bilirubin. </p> | ||
| − | <p>The part seems to work as intended. It has a histidine tag, expresses UnaG, and fluoresces when cloned into cells that are subsequently exposed to bilirubin</p> | + | <p>The part seems to work as intended. It has a histidine tag, expresses UnaG, and fluoresces when cloned into cells that are subsequently exposed to bilirubin </p> |
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Revision as of 14:50, 13 October 2018
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2669000
iGEM Uppsala 2018 Experience
After transforming our cells with a low copy amplicilin plasmid containing this composite part, cell lysis and affinity chromotography were used to extract UnaG from our cells. Please note: The exact procedure can be found at the end of our UnaG wiki page. Conducting "bilirubin tests" (the addition of a small amount of bilirubin dissolved in chloroform to samples) allowed us to see if UnaG was present in our samples, since as mentioned earlier UnaG fluoresces in the presence of bilirubin.The part seems to work as intended. It has a histidine tag, expresses UnaG, and fluoresces when cloned into cells that are subsequently exposed to bilirubin
===User Reviews===