Difference between revisions of "Part:BBa K2623023"
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It’s another form of the <partinfo>BBa_K2623021</partinfo> designed to target our archaeal ribosomal protein protein L7Ae and then siRNA into outer-membrane vesicles (OMVs). The only difference is that we inserted SpyTag to the C-termini of OmpA protein(instead of the N-termi ni <partinfo>BBa_K2623021</partinfo>). As mentioned in <partinfo>BBa_K2623021</partinfo>, SpyTag was constructed in order to anchore L7Ae to outer membrane through the bioconjugation of the SpyTag/SpyCatcher system. Through the fluorescence intensity of GFP, we can compare and evaluate expression efficiency of SpyTag between the <partinfo>BBa_K2623021</partinfo> and this part. Then the better one would be uesd to construct our final gene circuit. | It’s another form of the <partinfo>BBa_K2623021</partinfo> designed to target our archaeal ribosomal protein protein L7Ae and then siRNA into outer-membrane vesicles (OMVs). The only difference is that we inserted SpyTag to the C-termini of OmpA protein(instead of the N-termi ni <partinfo>BBa_K2623021</partinfo>). As mentioned in <partinfo>BBa_K2623021</partinfo>, SpyTag was constructed in order to anchore L7Ae to outer membrane through the bioconjugation of the SpyTag/SpyCatcher system. Through the fluorescence intensity of GFP, we can compare and evaluate expression efficiency of SpyTag between the <partinfo>BBa_K2623021</partinfo> and this part. Then the better one would be uesd to construct our final gene circuit. | ||
Revision as of 14:44, 13 October 2018
Bacterial outer membrane protein A (OmpA) fused with SpyTag and GFP at its N-termini
Summary
It’s another form of the BBa_K2623021 designed to target our archaeal ribosomal protein protein L7Ae and then siRNA into outer-membrane vesicles (OMVs). The only difference is that we inserted SpyTag to the C-termini of OmpA protein(instead of the N-termi ni BBa_K2623021). As mentioned in BBa_K2623021, SpyTag was constructed in order to anchore L7Ae to outer membrane through the bioconjugation of the SpyTag/SpyCatcher system. Through the fluorescence intensity of GFP, we can compare and evaluate expression efficiency of SpyTag between the BBa_K2623021 and this part. Then the better one would be uesd to construct our final gene circuit.
OTHER INFORMATION
You can visit the Results page: http://2018.igem.org/Team:XMU-China/Results to see the results of comparison of two reporters (BBa_K2623021 and BBa_K2623023)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 65
- 1000COMPATIBLE WITH RFC[1000]