Difference between revisions of "Part:BBa K2623028"

Revision as of 14:34, 13 October 2018

A composite part linked by SpyTag-OmpA and SpyCatcher-L7Ae-mRFP1

Summary

This part is a composite part constructed by linking BBa_K2623022 and BBa_K2623025 together. BBa_K2623022 can express OmpA inserted with SpyTag (ST) at its N-termini. BBa_K2623025 can express archaeal ribosomal protein protein L7Ae inserted with SpyCatcher (SC) at its N-termini and mRFP1 at its C-termini under the induction of IPTG at 0.5 mM concentration. It's another form of BBa_K2623030 with SpyTag inserted in the C-termini of OmpA.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1965
Illegal NheI site found at 1988
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 65
Illegal AgeI site found at 3122
Illegal AgeI site found at 3234
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2623028 short</partinfo>
+===Summary===
+This part is a composite part constructed by linking <partinfo>BBa_K2623022</partinfo> and <partinfo>BBa_K2623025</partinfo> together. <partinfo>BBa_K2623022</partinfo> can express OmpA inserted with SpyTag (ST) at its N-termini.  <partinfo>BBa_K2623025</partinfo> can express archaeal ribosomal protein protein L7Ae inserted with SpyCatcher (SC) at its N-termini and mRFP1 at its C-termini under the induction of IPTG at 0.5 mM concentration. It's another form of <partinfo>BBa_K2623030</partinfo> with SpyTag inserted in the C-termini of OmpA.
-Under the induction of IPTG, this part can code SpyTag-OmpA and SpyCatcher-L7Ae-RFP to be encapsulated into OMVs through the isopeptide bond formed between SpyTag/SpyCatche.
 <!-- Add more about the biology of this part here