Difference between revisions of "Part:BBa K2587009"

Revision as of 09:17, 13 October 2018

ptxD_opt

Most of the microorganisms, especially common used model organsims like Escherichia coli or Saccharomyces cerevisiae grow only very slow on phosphite as phosphorus source. Moreover in industry is not widely accepted to use antibiotic resistances as markers. Therefore a system is required, which allows avoidance of contamination by other microorganisms and at the same time represents a reliable selection marker. We present the use of the ptxD gene from Pseudomonas stutzeri together with a phosphite media (reduces growth of contaminants), which could abolish the use of antibiotics in the future and the more efficient use of a phosphorus source in the medium. In this case ptxD is codon optimised for S.cerevisiae.

• ptxD codes for phosphonate dehydrogenase
• oxidation of phosphite (phosphonate) using NAD+ and H₂0 to phosphate and NADH
• Selection marker for budding and fission yeast
• phosphite-oxidizing ability
• environmentally safe culture
• antibiotic free system
• pH optimum: 7.25- 7.75