Difference between revisions of "Part:BBa K2559000"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | Bacterial cellulose synthase Z (BcsZ) from several bacteria have been shown to possess an endo-β-1,4-glucanase (cellulase) activity, and E. coli protein was even crystallized in a complex with the substrate cellopentaose(Figure 2), however, the roles in cellulose biosynthesis have long remained obscure. | + | Bacterial cellulose synthase Z (BcsZ) from several bacteria have been shown to possess an endo-β-1,4-glucanase (cellulase) activity, and E. coli protein was even crystallized in a complex with the substrate cellopentaose(Figure 2), however, the roles in cellulose biosynthesis have long remained obscure.It's suspected that the BcsZ and BlgX may help the cell form the correct cellulose configuration through selective digestion. |
We introduced the Acetobacter xylinum bacterial cellulose synthase gene (including BcsZ, H, A, B, C, D, LX) into the model organism cyanobacteria (Synechocystis),and we obtain the transgenic cyanobacteria(Figure 3). | We introduced the Acetobacter xylinum bacterial cellulose synthase gene (including BcsZ, H, A, B, C, D, LX) into the model organism cyanobacteria (Synechocystis),and we obtain the transgenic cyanobacteria(Figure 3). | ||
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===The proposed organization of BcsZ in the BCS complexes === | ===The proposed organization of BcsZ in the BCS complexes === | ||
Revision as of 08:08, 13 October 2018
Bacterial cellulose synthase Z (BcsZ)
The BBa_K2559000 is an endoglucanase (Bcs Z) involved in the process of cellulose hydrolysis, and its roles in cellulose biosynthesis have long remained obscure.
Usage and Biology
Bacterial cellulose synthase Z (BcsZ) from several bacteria have been shown to possess an endo-β-1,4-glucanase (cellulase) activity, and E. coli protein was even crystallized in a complex with the substrate cellopentaose(Figure 2), however, the roles in cellulose biosynthesis have long remained obscure.It's suspected that the BcsZ and BlgX may help the cell form the correct cellulose configuration through selective digestion. We introduced the Acetobacter xylinum bacterial cellulose synthase gene (including BcsZ, H, A, B, C, D, LX) into the model organism cyanobacteria (Synechocystis),and we obtain the transgenic cyanobacteria(Figure 3).
The proposed organization of BcsZ in the BCS complexes
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 252