Difference between revisions of "Part:BBa K2637005:Design"
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===Source=== | ===Source=== | ||
| − | + | CikA is from the the cyanobacterium Synechococcus elongatus PCC7942 and naturally controls the phosphorylation of RpaA. In vivo, CikA will bind to the KaiBC complex during the subjective night and dephosphorylate RpaA via its phosphatase activities. | |
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===References=== | ===References=== | ||
Latest revision as of 06:44, 13 October 2018
CikA Protein of Circadian Rhythm
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 127
Illegal BamHI site found at 2187 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We performed codon optimization on this gene sequence to use it in yeast. And then we modified some sites to deleted some enzyme cutting sites.
Source
CikA is from the the cyanobacterium Synechococcus elongatus PCC7942 and naturally controls the phosphorylation of RpaA. In vivo, CikA will bind to the KaiBC complex during the subjective night and dephosphorylate RpaA via its phosphatase activities.