Difference between revisions of "Part:BBa K2559000"

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===Transcriptional The proposed organization of BcsZ in the BCS complexes ===
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===The proposed organization of BcsZ in the BCS complexes ===
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 03:11, 13 October 2018


Bacterial cellulose synthase Z (BcsZ) The BBa_K2559000 is an endoglucanase (Bcs Z) involved in the process of cellulose hydrolysis, and its roles in cellulose biosynthesis have long remained obscure.

Usage and Biology

Bacterial cellulose synthase Z (BcsZ) from several bacteria have been shown to possess an endo-β-1,4-glucanase (cellulase) activity, and E. coli protein was even crystallized in a complex with the substrate cellopentaose(Figure 2), however, the roles in cellulose biosynthesis have long remained obscure. We suspect that the BcsZ and BlgX may help the cell form the correct cellulose configuration through selective digestion. We introduced the Acetobacter xylinum bacterial cellulose synthase gene (including BcsZ, H, A, B, C, D, LX) into the model organism cyanobacteria (Synechocystis),and we obtain the transgenic cyanobacteria(Figure 3).


The proposed organization of BcsZ in the BCS complexes

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 252